BLAST Search

Query Comment (optional, will be added to output for your use):

NOTE: If the input sequence is less than 30 letters you should change the default Cutoff Score value to something less than 100 or you can miss matches.

Upload Local TEXT File: FASTA, GCG, and RAW sequence formats are okay
WORD Documents do not work unless saved as TEXT.

Type or Paste a Query Sequence : (No Comments, Numbers Okay)

Choose the Appropriate BLAST Program:

Choose a Sequence Database:


Options: For descriptions of BLAST options and parameters, refer to the BLAST documentation at NCBI.

Do not change the Matrix (BLOSUM62) if you blast against a nucleotide Database!

Output format :
Comparison Matrix :
Cutoff Score (S value) :
Word Length (W value) :Default = 11 for BLASTN, 3 for all others
Expect threshold (E threshold) :
Number of best alignments to show :
Filter options : DUST file for BLASTN, SEQ filter for all others